Volatile-sulfur-compound profile distinguishes Burkholderia pseudomallei from Burkholderia thailandensis

Timothy J J Inglis; Dorothee R Hahne; Adam J Merritt; Michael W Clarke

doi:10.1128/JCM.03644-14

Volatile-sulfur-compound profile distinguishes Burkholderia pseudomallei from Burkholderia thailandensis

J Clin Microbiol. 2015 Mar;53(3):1009-11. doi: 10.1128/JCM.03644-14. Epub 2015 Jan 7.

Authors

Timothy J J Inglis¹, Dorothee R Hahne², Adam J Merritt³, Michael W Clarke²

Affiliations

¹ School of Pathology and Laboratory Medicine, The University of Western Australia, Crawley, WA, Australia Department of Microbiology, PathWest Laboratory Medicine, Nedlands, WA, Australia tim.inglis@health.wa.gov.au.
² UWA Centre for Metabolomics, The University of Western Australia, Crawley, WA, Australia.
³ School of Pathology and Laboratory Medicine, The University of Western Australia, Crawley, WA, Australia Department of Microbiology, PathWest Laboratory Medicine, Nedlands, WA, Australia.

Abstract

Solid-phase microextraction gas chromatography-mass spectrometry (SPME-GCMS) was used to show that dimethyl sulfide produced by Burkholderia pseudomallei is responsible for its unusual truffle-like smell and distinguishes the species from Burkholderia thailandensis. SPME-GCMS can be safely used to detect dimethyl sulfide produced by agar-grown B. pseudomallei.

MeSH terms

Agar
Bacteriological Techniques / methods*
Burkholderia / chemistry*
Burkholderia / classification*
Burkholderia / growth & development
Culture Media / chemistry
Gas Chromatography-Mass Spectrometry / methods*
Sulfides / analysis*
Volatile Organic Compounds / analysis*

Substances

Culture Media
Sulfides
Volatile Organic Compounds
Agar
dimethyl sulfide