Objective: To investigate the constituents with antioxidant activities from alcalase hydrolysate of Arca subcrenata.
Methods: The consecutive chromatographic methods were employed,including ion-exchange chromatography, gel filtration chromatography, and reverse phase high-performance liquid chromatography (RP-HPLC). The amino acid sequences of the purified antioxidant peptides were determined by automated Edman degradation.
Results: Under the guidance of the assay of scavenging free radicals, two peptides with antioxidant activities, termed as A-Bg1 and A-Bh, were isolated and purified from the alcalase hydrolysate of Arca subcrenata.
Conclusion: Constituents from the hydrolysate of Arca subcrenata might be a new potential resource of antioxidants.