Recombinant protein (EGFP-Protein G)-coated PbS quantum dots for in vitro and in vivo dual fluorescence (visible and second-NIR) imaging of breast tumors

Nanoscale. 2015 Mar 12;7(12):5115-9. doi: 10.1039/c4nr06480a.

Abstract

We report a one-step synthetic strategy for the preparation of recombinant protein (EGFP-Protein G)-coated PbS quantum dots for dual (visible and second-NIR) fluorescence imaging of breast tumors at the cellular and whole-body level.

MeSH terms

  • Animals
  • Breast Neoplasms / metabolism*
  • Breast Neoplasms / pathology*
  • Cell Line, Tumor
  • Coated Materials, Biocompatible / chemical synthesis
  • Green Fluorescent Proteins / chemistry*
  • Green Fluorescent Proteins / genetics
  • Humans
  • Image Enhancement / methods
  • Infrared Rays
  • Lead / chemistry*
  • Lead / radiation effects
  • Mice
  • Mice, Nude
  • Microscopy, Fluorescence, Multiphoton / methods*
  • Quantum Dots*
  • Recombinant Proteins / pharmacokinetics
  • Reproducibility of Results
  • Selenium Compounds / chemistry*
  • Selenium Compounds / radiation effects
  • Sensitivity and Specificity
  • Whole Body Imaging / methods

Substances

  • Coated Materials, Biocompatible
  • Recombinant Proteins
  • Selenium Compounds
  • enhanced green fluorescent protein
  • lead selenide
  • Green Fluorescent Proteins
  • Lead