Objective: To determine the high performance liquid chromatography(HPLC)characteristic spectrum of artificial musk.
Methods: HPLC was performed on a HiQ Sil C18 analytical column(4.6 mm×250 mm)with the mobile phase of methanol/water(90:10 v/v);the detection wavelength was 254 nm and the column temperature was maintained at 25 ℃. The similarities among 10 batches of artificial musk samples were anlysised in accordance with the System for Evaluating the Similarities among the Chromatographic Fingerprints of Traditional Chinese Drugs,which is recommended by Chinese Pharmacopoeia Commission.
Result: The similarities among these 10 batches of artificial musk ranged between 0.999 and 1.000.
Conclusion: HPLC is a convenient,accurate and reliable technique for establishing the specific spectum of artificial musk and therefore can be used for the quality control of this product.