Rapid evaluation of N-glycosylation status of antibodies with chemiluminescent lectin-binding assay

Masayoshi Onitsuka; Takeshi Omasa

doi:10.1016/j.jbiosc.2014.11.015

Rapid evaluation of N-glycosylation status of antibodies with chemiluminescent lectin-binding assay

J Biosci Bioeng. 2015 Jul;120(1):107-10. doi: 10.1016/j.jbiosc.2014.11.015. Epub 2014 Dec 23.

Authors

Masayoshi Onitsuka¹, Takeshi Omasa²

Affiliations

¹ Institute of Technology and Science, Tokushima University, Minamijosanjima-cho 2-1, Tokushima 770-8506, Japan. Electronic address: onitsuka@tokushima-u.ac.jp.
² Institute of Technology and Science, Tokushima University, Minamijosanjima-cho 2-1, Tokushima 770-8506, Japan.

PMID: 25548123
DOI: 10.1016/j.jbiosc.2014.11.015

Abstract

We constructed an assay system for the rapid detection of the glycosylation status of antibodies with a chemiluminescence-based lectin-binding assay, and investigated the glycosylation dynamics during the culture of recombinant Chinese hamster ovary cells. This rapid detection system is applicable to the high-throughput evaluation of therapeutic glycoproteins.

Keywords: Antibody; Chemiluminescent assay; Chinese hamster ovary cells; Glycosylation; Lectin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies / chemistry*
Antibodies / metabolism*
CHO Cells
Cricetulus
Glycoproteins / analysis
Glycoproteins / chemistry*
Glycoproteins / metabolism*
Glycosylation
Humans
Lectins / metabolism*
Luminescence
Luminescent Measurements*
Protein Binding
Time Factors

Substances

Antibodies
Glycoproteins
Lectins