Use of an exposure chamber to maintain aqueous phase nanoparticle dispersions for improved toxicity testing in fish

David Boyle; Halis Boran; Andrew J Atfield; Theodore B Henry

doi:10.1002/etc.2840

Use of an exposure chamber to maintain aqueous phase nanoparticle dispersions for improved toxicity testing in fish

Environ Toxicol Chem. 2015 Mar;34(3):583-8. doi: 10.1002/etc.2840. Epub 2015 Jan 30.

Authors

David Boyle¹, Halis Boran, Andrew J Atfield, Theodore B Henry

Affiliation

¹ School of Biomedical and Biological Sciences, Plymouth University, Plymouth, Devon, United Kingdom.

PMID: 25545389
DOI: 10.1002/etc.2840

Abstract

A novel chamber for maintaining aqueous phase dispersions of nanoparticles (NPs) to enable improved toxicity testing in larval zebrafish was developed. Aqueous concentrations were within 80% of initial NP concentrations, and the 96-h median lethal concentration (LC50) values were highly reproducible (coefficient of variation <0.16, n = 3 tests). Significantly lower toxicity for each NP tested (Ag, Cu, and TiO2 NPs) in static beakers suggested that traditional acute toxicity tests may underestimate aqueous phase toxicity of NPs.

Keywords: Acute toxicity; Danio rerio; Engineered nanoparticles; Nanoparticle exposure chamber.

MeSH terms

Animals
Environmental Exposure / analysis*
Larva / drug effects
Nanoparticles / toxicity*
Particle Size
Silver / analysis
Toxicity Tests / methods*
Toxicity Tests, Acute
Zebrafish / physiology*

Substances

Silver