Effects of detyrosinated tubulin on Na+,K+-ATPase activity and erythrocyte function in hypertensive subjects

FEBS Lett. 2015 Jan 30;589(3):364-73. doi: 10.1016/j.febslet.2014.12.022. Epub 2014 Dec 23.

Abstract

Formation of tubulin/Na(+),K(+)-ATPase (NKA) complex in erythrocytes of hypertensive subjects results in a 50% reduction in NKA activity. We demonstrate here that detyrosinated tubulin, which is increased in hypertensive erythrocytes membranes, enhances the inhibitory effect of acetylated tubulin on NKA activity. Moreover, we report a reduced content and activity of the enzyme tubulin tyrosine ligase in erythrocytes of hypertensive subjects. Such alterations are related to changes in erythrocyte deformability. Our findings indicate that the detyrosination/tyrosination cycle of tubulin is important in regulation of NKA activity, and that abnormalities in this cycle are involved in hypertension development.

Keywords: Detyrosinated tubulin; Erythrocyte deformability; Human erythrocytes; Hypertension; Na(+),K(+)-ATPase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Erythrocyte Deformability / genetics
  • Erythrocytes / enzymology*
  • Erythrocytes / pathology
  • Female
  • Humans
  • Hypertension / genetics
  • Hypertension / metabolism*
  • Hypertension / pathology
  • Male
  • Middle Aged
  • Sodium-Potassium-Exchanging ATPase / genetics
  • Sodium-Potassium-Exchanging ATPase / metabolism*
  • Tubulin / metabolism*
  • Tyrosine / metabolism

Substances

  • Tubulin
  • Tyrosine
  • Sodium-Potassium-Exchanging ATPase