MiR-378a-3p enhances adipogenesis by targeting mitogen-activated protein kinase 1

Biochem Biophys Res Commun. 2015 Jan 30;457(1):37-42. doi: 10.1016/j.bbrc.2014.12.055. Epub 2014 Dec 18.

Abstract

Previous studies showed that miR-378a plays important roles in adipogenesis and obesity; however, the precise mechanisms of action remain unknown. Here, we found that miR-378a-3p expression is up-regulated in adipose tissues of high fat diet-induced obese mice, as well as during the differentiation of 3T3-L1 preadipocytes. Mir-378a-3p induced adipogenesis by targeting mitogen-activated protein kinase 1 (MAPK1). Overexpression of miR-378a-3p or silencing MAPK1 reduced MAPK1 expression and enhanced adipogenesis, whereas blockage of endogenous miR-378a-3p had the opposite effect, suggesting that miR-378a-3p promotes the adipogenesis of 3T3-L1 cells by targeting MAPK1.

Keywords: Adipogenesis; MAPK1; MiR-378a-3p.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3-L1 Cells
  • Adipogenesis* / genetics
  • Animals
  • Base Sequence
  • Diet, High-Fat
  • Male
  • Mice
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • Mitogen-Activated Protein Kinase 1 / metabolism*
  • Models, Biological
  • Molecular Sequence Data
  • Obesity / genetics
  • Obesity / pathology
  • Up-Regulation / genetics

Substances

  • MIRN378 microRNA, mouse
  • MicroRNAs
  • Mitogen-Activated Protein Kinase 1