Abstract
The POM-1 metallo-β-lactamase is a subclass B3 resident enzyme produced by Pseudomonas otitidis, a pathogen causing otic infections. The enzyme was overproduced in Escherichia coli BL21(DE3), purified by chromatography, and subjected to structural and functional analysis. The purified POM-1 is a tetrameric enzyme of broad substrate specificity with higher catalytic activities with penicillins and carbapenems than with cephalosporins.
Copyright © 2015, American Society for Microbiology. All Rights Reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / pharmacology
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Bacterial Proteins / metabolism*
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Bacterial Proteins / pharmacology*
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Carbapenems / pharmacology
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Catalysis
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Cephalosporins / pharmacology
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Escherichia coli / metabolism
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Penicillins / pharmacology
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Pseudomonas / enzymology*
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Pseudomonas / metabolism*
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beta-Lactamases / metabolism*
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beta-Lactamases / pharmacology*
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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Carbapenems
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Cephalosporins
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Penicillins
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beta-Lactamases