Diffraction optical tomography using a quantitative phase imaging unit

Opt Lett. 2014 Dec 15;39(24):6935-8. doi: 10.1364/OL.39.006935.

Abstract

A simple and practical method to measure three-dimensional (3-D) refractive index (RI) distributions of biological cells is presented. A common-path self-reference interferometry consisting of a compact set of polarizers is attached to a conventional inverted microscope equipped with a beam scanning unit, which can precisely measure multiple 2-D holograms of a sample with high phase stability for various illumination angles, from which accurate 3-D optical diffraction tomograms of the sample can be reconstructed. 3-D RI tomograms of nonbiological samples such as polystyrene microspheres, as well as biological samples including human red blood cells and breast cancer cells, are presented.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Erythrocytes / cytology
  • Humans
  • Microscopy
  • Polystyrenes
  • Tomography, Optical / methods*

Substances

  • Polystyrenes