The synthesis of UDP-selective fluorescent probe and its imaging application in living cells

Srinivasulu Kambam; Xintong Ren; Cheng Zheng; Fang Wang; Yong Wang; Haiyan Chen; Jun Yin; Jingjing Xie; Xiaoqiang Chen

doi:10.1016/j.bmcl.2014.11.057

The synthesis of UDP-selective fluorescent probe and its imaging application in living cells

Bioorg Med Chem Lett. 2015 Jan 15;25(2):262-5. doi: 10.1016/j.bmcl.2014.11.057. Epub 2014 Nov 27.

Authors

Srinivasulu Kambam¹, Xintong Ren¹, Cheng Zheng², Fang Wang¹, Yong Wang¹, Haiyan Chen³, Jun Yin⁴, Jingjing Xie², Xiaoqiang Chen⁵

Affiliations

¹ State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemistry and Chemical Engineering, Nanjing Tech University, Nanjing 210009, China.
² State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, China.
³ Department of Biomedical Engineering, School of Life Science and Technology, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China. Electronic address: chenhaiyan@cpu.edu.cn.
⁴ Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, China.
⁵ State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemistry and Chemical Engineering, Nanjing Tech University, Nanjing 210009, China. Electronic address: chenxq@njtech.edu.cn.

PMID: 25499877
DOI: 10.1016/j.bmcl.2014.11.057

Abstract

A perylene-based probe was developed for uridine diphosphate (UDP) sensing and cell imaging. The probe presented about 4-fold fluorescence enhancement in the presence or absence of 100equiv UDP. The selectivity toward UDP over other phosphor-containing anions was observed. The selective UDP sensing was speculated to be related to the binding affinities of Zn(2+) ions in sensor with the uridine and phosphate moieties of UDP. Furthermore, this probe was also applied to image of UDP in living cells.

Keywords: Cell imaging; Fluorescent probe; Perylene; UDP.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Breast Neoplasms / diagnosis*
Female
Fluorescence
Fluorescent Dyes / chemical synthesis*
Humans
Microscopy, Confocal
Molecular Structure
Perylene / chemistry
Spectrometry, Fluorescence
Structure-Activity Relationship
Tumor Cells, Cultured
Uridine Diphosphate / chemistry*
Zinc / chemistry

Substances

Fluorescent Dyes
Uridine Diphosphate
Perylene
Zinc