Development of liposomal Ganoderma lucidum polysaccharide: formulation optimization and evaluation of its immunological activity

Zhenguang Liu; Xia Ma; Bihua Deng; Yee Huang; Ruonan Bo; Zhenzhen Gao; Yun Yu; Yuanliang Hu; Jiaguo Liu; Yi Wu; Deyun Wang

doi:10.1016/j.carbpol.2014.09.093

Development of liposomal Ganoderma lucidum polysaccharide: formulation optimization and evaluation of its immunological activity

Carbohydr Polym. 2015 Mar 6:117:510-517. doi: 10.1016/j.carbpol.2014.09.093. Epub 2014 Oct 15.

Authors

Zhenguang Liu¹, Xia Ma², Bihua Deng³, Yee Huang¹, Ruonan Bo¹, Zhenzhen Gao¹, Yun Yu¹, Yuanliang Hu¹, Jiaguo Liu¹, Yi Wu¹, Deyun Wang⁴

Affiliations

¹ Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
² Henan University of Animal Husbandry & Economy, Zhengzhou 450011, PR China.
³ National Research Center of Veterinary Biologicals Engineering and Technology, Jiangsu Academy of Agricultural Science, Nanjing 210014, China.
⁴ Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: dywang@njau.edu.cn.

PMID: 25498665
DOI: 10.1016/j.carbpol.2014.09.093

Abstract

The aim of this study was to investigate the optimizing preparation conditions of Ganoderma lucidum polysaccharide liposome (GLPL) with response surface methodology (RSM) and the immunological enhancement activity of GLPL. The immunological enhancement activity of GLPL on splenocyte proliferation was measured. The optimum formulation of GLPL, in which the ratio of soybean phospholipid to cholesterol(w/w) of 11:1, the ratio of soybean phospholipid to tween-80 (w/w) of 10.5:1 and ultrasonic time(min) of 11, had higher entrapment efficiency (EE) of 71.43±0.49% with spherical shape and uniform sizes. In addition, GLPL could significantly promote splenocyte proliferation singly or synergistically with PHA and LPS. That indicated that the immunological enhancement of Ganoderma lucidum polysaccharide (GLP) was significantly enhanced after encapsulation with the liposome.

Keywords: Ganoderma lucidum polysaccharides liposome; Lymphocyte proliferation; Optimization; Response surface methodology.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Liposomes / chemistry*
Microscopy, Electron
Polysaccharides / chemistry*
Polysaccharides / immunology*
Reishi / chemistry*
Surface Properties

Substances

Liposomes
Polysaccharides