Isolation, molecular and phylogenetic analysis of encephalomyocarditis virus strain GS01 in China

Ruofei Feng; Haixia Zhang; Jia Wei; Xiangrong Li; Jingying Xie; Mingsheng Li; Zilin Qiao; Yuping Feng; Zhongren Ma

doi:10.1016/j.meegid.2014.12.004

Isolation, molecular and phylogenetic analysis of encephalomyocarditis virus strain GS01 in China

Infect Genet Evol. 2015 Mar:30:19-26. doi: 10.1016/j.meegid.2014.12.004. Epub 2014 Dec 9.

Authors

Ruofei Feng¹, Haixia Zhang², Jia Wei³, Xiangrong Li², Jingying Xie², Mingsheng Li⁴, Zilin Qiao⁴, Yuping Feng⁴, Zhongren Ma⁵

Affiliations

¹ Animal Cell Engineering & Technology Research Center of Gansu, Northwest University for Nationalities, No. 1 Xibeixincun, Lanzhou 730030, PR China; The Key Bio-engineering and Technology Laboratory of SEAC, Northwest University For Nationalities, No. 1 Xibeixincun, Lanzhou 730030, PR China.
² The Key Bio-engineering and Technology Laboratory of SEAC, Northwest University For Nationalities, No. 1 Xibeixincun, Lanzhou 730030, PR China.
³ Animal Cell Engineering & Technology Research Center of Gansu, Northwest University for Nationalities, No. 1 Xibeixincun, Lanzhou 730030, PR China; School of Life Science and Bioengineering, Northwest University for Nationalities, No. 1 Xibeixincun, Lanzhou 730030, PR China.
⁴ Animal Cell Engineering & Technology Research Center of Gansu, Northwest University for Nationalities, No. 1 Xibeixincun, Lanzhou 730030, PR China.
⁵ Animal Cell Engineering & Technology Research Center of Gansu, Northwest University for Nationalities, No. 1 Xibeixincun, Lanzhou 730030, PR China; The Key Bio-engineering and Technology Laboratory of SEAC, Northwest University For Nationalities, No. 1 Xibeixincun, Lanzhou 730030, PR China. Electronic address: mazhongren@xbmu.edu.cn.

PMID: 25497352
DOI: 10.1016/j.meegid.2014.12.004

Abstract

Encephalomyocarditis virus (EMCV) is a small non-enveloped, single-stranded RNA virus. It can infect many host species and cause acute myocarditis and respiratory failure in piglets, reproductive failure in pregnant sows. Diseases caused by EMCV currently affect the swine industry worldwide. In this study, an EMCV strain was isolated from an aborted fetus in western China. It was identified by reverse-transcription polymerase chain reaction (RT-PCR) and genome sequencing. The subsequent results showed that the virus could produce a specific cytopathic effect on BHK-21 cells and could cause severe clinical symptoms and pathological changes in mice. Complete genome sequencing and multiple sequence alignment indicated that the GS01 strain was 79.9-99.9% identical with other isolates worldwide. Phylogenetic analysis showed that EMCV isolates fell into five clusters: lineage 1, 2, 3, 4, and 5 based on the nucleotide sequences of the entire ORF and VP3/VP1 junction, as well as 3D gene. GS01 isolate was grouped into lineage 1. The results of this study confirmed that an EMCV strain GS01 isolated from an aborted pig fetus in western China was fatal to mice and provided new epidemiologic data on EMCV in China.

Keywords: EMCV; Isolation; Molecular characterization; Phylogenetic analysis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cardiovirus Infections / mortality
Cardiovirus Infections / veterinary
Cardiovirus Infections / virology*
Cell Line
China
Cricetinae
Encephalomyocarditis virus / classification*
Encephalomyocarditis virus / genetics*
Encephalomyocarditis virus / isolation & purification
Mice
Phylogeny
Swine / virology
Swine Diseases / virology*