Structure of the O-specific polysaccharide from a marine bacterium Echinicola vietnamensis KММ 6221Т

Svetlana V Tomshich; Maxim S Kokoulin; Anatoliy I Kalinovsky; Nadezhda A Komandrova; Ol'ga I Nedashkovskaya

doi:10.1016/j.carres.2014.05.001

Structure of the O-specific polysaccharide from a marine bacterium Echinicola vietnamensis KММ 6221Т

Carbohydr Res. 2015 Jan 30:402:1-5. doi: 10.1016/j.carres.2014.05.001. Epub 2014 May 14.

Authors

Svetlana V Tomshich¹, Maxim S Kokoulin², Anatoliy I Kalinovsky², Nadezhda A Komandrova², Ol'ga I Nedashkovskaya²

Affiliations

¹ G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, 690022 Vladivostok, Russian Federation. Electronic address: tomsh@piboc.dvo.ru.
² G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, 690022 Vladivostok, Russian Federation.

PMID: 25483030
DOI: 10.1016/j.carres.2014.05.001

Abstract

The O-specific polysaccharide was isolated from the lipopolysaccharide of Echinicola vietnamensis and studied by chemical methods along with 1H and 13C NMR spectroscopy, including 2D 1H, 1H COSY, TOCSY, ROESY, 1H, 13C HSQC, HMBC, and H2BC experiments. It was found that the polysaccharide is built up of branched tetrasaccharide repeating units, containing 2-acetamido-2-deoxy-D-glucuronic acid (GlcNAcA), 2-acetamido-2-deoxy-D-galactose (GalNAc), and D-glucuronic acid (GlcA) with a terminal residue of 3,6-dideoxy-L-xylo-hexose (colitose, Col) and has the following structure. α-Colp1-->2-->4)-β-D-GlcpNAcA-(1-->4)-β-D-GlcpA-(1-->3)-α-D-GalpNAc-(1-->

Keywords: 3,6-Dideoxy-l-xylo-hexose (Col); Bacterial polysaccharide structure; Echinicola vietnamensis; Marine bacterium; O-specific polysaccharide.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteroidetes / chemistry*
Carbohydrate Sequence
Magnetic Resonance Spectroscopy
Molecular Sequence Data
O Antigens / chemistry*

Substances

O Antigens