Digital pathology with Fourier ptychography

Comput Med Imaging Graph. 2015 Jun:42:38-43. doi: 10.1016/j.compmedimag.2014.11.005. Epub 2014 Nov 18.

Abstract

Fourier ptychographic microscopy (FPM) is a recently introduced method of acquiring high-resolution, wide field of view (FOV) giga-pixel histology images. The FPM procedure first acquires a sequence of low-resolution images of a sample under variable-angle illumination. It then combines these images using a novel phase retrieval algorithm to improve the employed microscope's resolution beyond its conventional limit. Here, we first describe how FPM's resolution improvement can enhance wide FOV histology imaging. Second, we show that FPM also records a thin sample's optical phase, which can help pathologists digitally extract as much information as possible from a given histology slide.

Keywords: Computational imaging; Digital pathology; Microscopy; Structured illumination; Super-resolution.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms*
  • Equipment Design
  • Equipment Failure Analysis
  • Fourier Analysis
  • Histological Techniques / instrumentation
  • Histological Techniques / methods*
  • Image Enhancement / methods
  • Image Interpretation, Computer-Assisted / methods*
  • Lighting / instrumentation
  • Lighting / methods*
  • Microscopy, Phase-Contrast / instrumentation
  • Microscopy, Phase-Contrast / methods*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Signal Processing, Computer-Assisted