Hypoxic X-irradiation as an external stimulus for conformational change of oligodeoxynucleotides that possess disulfide bond and regulation of DNAzyme function

Bioorg Med Chem Lett. 2015 Jan 15;25(2):310-2. doi: 10.1016/j.bmcl.2014.11.043. Epub 2014 Nov 22.

Abstract

We achieved a conformational change of oligodeoxynucleotides and the regulation of DNAzyme function by means of a radiolytic strand exchange reaction of disulfide bond. We designed a system in which the DNAzyme function of RNA cleavage was suppressed by the hybridization of an inhibitor strand that possessed disulfide bond with an active DNAzyme. Hypoxic X-irradiation led to the recovery of RNA cleavage because the strand exchange reaction at the disulfide bond in inhibitor strand resulted in a release of inhibitor strand. This strategy may be applicable to gene regulation by hypoxic X-irradiation.

Keywords: DNAzyme; Disulfide bond; Hypoxic X-irradiation; Oligodeoxynucleotides.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA, Catalytic / chemistry*
  • DNA, Catalytic / metabolism*
  • Disulfides / chemistry*
  • Humans
  • Hypoxia*
  • Nucleic Acid Conformation
  • Oligodeoxyribonucleotides / chemistry*
  • Oligodeoxyribonucleotides / radiation effects*
  • Oxidation-Reduction
  • X-Rays

Substances

  • DNA, Catalytic
  • Disulfides
  • Oligodeoxyribonucleotides