Rapid determination of total polyphenolic content in tea samples based on caffeic acid voltammetric behaviour on a disposable graphite electrode

Iulia Gabriela David; Ana-Maria Cristina Bizgan; Dana Elena Popa; Mihaela Buleandra; Zenovia Moldovan; Irinel Adriana Badea; Tuğçe Ayça Tekiner; Huveyda Basaga; Anton A Ciucu

doi:10.1016/j.foodchem.2014.10.139

Rapid determination of total polyphenolic content in tea samples based on caffeic acid voltammetric behaviour on a disposable graphite electrode

Food Chem. 2015 Apr 15:173:1059-65. doi: 10.1016/j.foodchem.2014.10.139. Epub 2014 Nov 3.

Authors

Iulia Gabriela David¹, Ana-Maria Cristina Bizgan², Dana Elena Popa², Mihaela Buleandra², Zenovia Moldovan², Irinel Adriana Badea², Tuğçe Ayça Tekiner³, Huveyda Basaga³, Anton A Ciucu²

Affiliations

¹ Department of Analytical Chemistry, Faculty of Chemistry, University of Bucharest, 4-12 Regina Elisabeta Av., 030018, District 3, Bucharest, Romania. Electronic address: gabrielaiulia.david@g.unibuc.ro.
² Department of Analytical Chemistry, Faculty of Chemistry, University of Bucharest, 4-12 Regina Elisabeta Av., 030018, District 3, Bucharest, Romania.
³ Biological Sciences and Bioengineering Program, Sabanci University, 34457 Orhanlı-Tuzla, Istanbul, Turkey.

PMID: 25466125
DOI: 10.1016/j.foodchem.2014.10.139

Abstract

The present paper describes the voltammetric behaviour and the quantitative determination of caffeic acid (CA) on a disposable pencil graphite electrode (PGE). The anodic peak current of CA recorded by differential pulse voltammetry (DPV) varies linearly with CA concentration in the range 1×10(-7)-3×10(-3) M. The detection and quantification limits were 8.83×10(-8) M and 2.94×10(-7) M caffeic acid, respectively. The mean recoveries of CA from Turkish green, white and black teas were 98.30%, 99.57% and 91.46%. For these three tea types the corresponding total polyphenolic contents (TPCs) evaluated by DPV on PGE were 35.81, 34.59 and 31.21 mg caffeic acid equivalent/g tea, respectively. These TPC values were in good accordance with those obtained by the Folin-Ciocalteu method. The developed DPV on PGE method constitutes a simple and inexpensive tool for the rapid assessment of TPC of tea samples.

Keywords: Caffeic acid; Pencil graphite electrode; Tea; Total polyphenolic content.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Caffeic Acids / chemistry*
Electrochemical Techniques / instrumentation*
Electrochemical Techniques / methods
Electrodes*
Graphite*
Polyphenols / analysis*
Tea / chemistry*

Substances

Caffeic Acids
Polyphenols
Tea
Graphite
caffeic acid