Multifamily determination of pesticide residues in soya-based nutraceutical products by GC/MS-MS

Agneša Páleníková; Gerardo Martínez-Domínguez; Francisco Javier Arrebola; Roberto Romero-González; Svetlana Hrouzková; Antonia Garrido Frenich

doi:10.1016/j.foodchem.2014.10.100

Multifamily determination of pesticide residues in soya-based nutraceutical products by GC/MS-MS

Food Chem. 2015 Apr 15:173:796-807. doi: 10.1016/j.foodchem.2014.10.100. Epub 2014 Oct 27.

Authors

Agneša Páleníková¹, Gerardo Martínez-Domínguez², Francisco Javier Arrebola², Roberto Romero-González², Svetlana Hrouzková³, Antonia Garrido Frenich⁴

Affiliations

¹ Research Group "Analytical Chemistry of Contaminants", Department of Chemistry and Physics, Research Centre for Agricultural and Food Biotechnology (BITAL), University of Almería, Agrifood Campus of International Excellence, ceiA3, E-04120 Almería, Spain; Institute of Analytical Chemistry, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, Bratislava 812 37, Slovak Republic.
² Research Group "Analytical Chemistry of Contaminants", Department of Chemistry and Physics, Research Centre for Agricultural and Food Biotechnology (BITAL), University of Almería, Agrifood Campus of International Excellence, ceiA3, E-04120 Almería, Spain.
³ Institute of Analytical Chemistry, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, Bratislava 812 37, Slovak Republic.
⁴ Research Group "Analytical Chemistry of Contaminants", Department of Chemistry and Physics, Research Centre for Agricultural and Food Biotechnology (BITAL), University of Almería, Agrifood Campus of International Excellence, ceiA3, E-04120 Almería, Spain. Electronic address: agarrido@ual.es.

PMID: 25466092
DOI: 10.1016/j.foodchem.2014.10.100

Abstract

An analytical method based on a modified QuEChERS extraction coupled with gas chromatography-tandem mass spectrometry (GC-MS/MS) was evaluated for the determination of 177 pesticides in soya-based nutraceutical products. The QuEChERS method was optimised and different extraction solvents and clean-up approaches were tested, obtaining the most efficient conditions with a mixture of sorbents (PSA, C18, GBC and Zr-Sep(+)). Recoveries were evaluated at 10, 50 and 100 μg/kg and ranged between 70% and 120%. Precision was expressed as relative standard deviation (RSD), and it was evaluated for more than 160 pesticides as intra and inter-day precision, with values always below 20% and 25%, respectively. Limits of detection (LODs) ranged from 0.1 to 10 μg/kg, whereas limits of quantification (LOQs) from 0.5 to 20 μg/kg. The applicability of the method was proved by analysing soya-based nutraceuticals. Two pesticides were found in these samples, malathion and pyriproxyfen, at 11.1 and 1.5 μg/kg respectively.

Keywords: GC–QqQ-MS/MS; Pesticide residues; QuEChERS; Soya nutraceuticals.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Dietary Supplements / analysis*
Food Analysis / methods
Gas Chromatography-Mass Spectrometry / methods*
Glycine max*
Pesticide Residues / analysis*
Tandem Mass Spectrometry / methods*

Substances

Pesticide Residues