Pectic polysaccharide from the green fruits of Momordica charantia (Karela): structural characterization and study of immunoenhancing and antioxidant properties

Carbohydr Res. 2015 Jan 12:401:24-31. doi: 10.1016/j.carres.2014.10.015. Epub 2014 Oct 25.

Abstract

A water soluble pectic polysaccharide (PS) isolated from the aqueous extract of the green fruits of Momordica charantia contains D-galactose and D-methyl galacturonate in a molar ratio of nearly 1:4. It showed splenocyte, thymocyte as well as macrophage activations. Moreover, it exhibited potent antioxidant activities. On the basis of total acid hydrolysis, methylation analysis, periodate oxidation, and 1D and 2D NMR studies, the structure of the repeating unit of the pectic polysaccharide was established as: [Formula: see text].

Keywords: Antioxidant activities; Immunoactivation; Momordica charantia; NMR studies; Pectic polysaccharide; Structure.

MeSH terms

  • Adjuvants, Immunologic / chemistry
  • Adjuvants, Immunologic / isolation & purification
  • Adjuvants, Immunologic / pharmacology
  • Animals
  • Antioxidants / chemistry
  • Antioxidants / isolation & purification
  • Antioxidants / pharmacology
  • Carbohydrate Sequence
  • Cell Proliferation / drug effects
  • Fruit / chemistry*
  • Hydroxyl Radical / chemistry
  • Lipid Peroxidation / drug effects
  • Mice
  • Molecular Sequence Data
  • Momordica charantia / chemistry*
  • Pectins / chemistry*
  • Pectins / isolation & purification
  • Pectins / pharmacology*
  • Spleen / cytology
  • Spleen / drug effects
  • Thymocytes / cytology
  • Thymocytes / drug effects

Substances

  • Adjuvants, Immunologic
  • Antioxidants
  • Hydroxyl Radical
  • Pectins