A simplified method for simultaneous detection of Rice stripe virus and Rice black-streaked dwarf virus in insect vector

Shuo Li; Xi Wang; Jianxiang Xu; Yinghua Ji; Yijun Zhou

doi:10.1016/j.jviromet.2014.10.006

A simplified method for simultaneous detection of Rice stripe virus and Rice black-streaked dwarf virus in insect vector

J Virol Methods. 2015 Jan:211:32-5. doi: 10.1016/j.jviromet.2014.10.006. Epub 2014 Oct 25.

Authors

Shuo Li¹, Xi Wang², Jianxiang Xu³, Yinghua Ji¹, Yijun Zhou⁴

Affiliations

¹ Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing 210014, China.
² Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing 210014, China; College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China.
³ College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China.
⁴ Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing 210014, China. Electronic address: yjzhou@jaas.ac.cn.

PMID: 25455902
DOI: 10.1016/j.jviromet.2014.10.006

Abstract

Rice stripe virus (RSV) and Rice black-streaked dwarf virus (RBSDV) are transmitted by their common vector small brown planthopper (SBPH) that cause serious crop losses in China. A simple reverse transcription-PCR method was developed for the simultaneous detection of RSV and RBSDV in single SBPH. Three primers targeted to RSV-RNA4 and RBSDV-S2 segments were designed to amplify respectively 1114-bp and 414-bp fragments in a reaction. The method is reliable, rapid and inexpensive for detecting the two viruses in vector, which could facilitate better forecasting and control of the virus diseases. Using this method, it was found that SBPH could carry RSV and RBSDV simultaneously.

Keywords: Rice black-streaked dwarf virus; Rice stripe virus; Simultaneous detection via RT-PCR.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
China
Costs and Cost Analysis
Entomology / methods*
Hemiptera / virology*
Insect Vectors / virology*
Oryza / virology
Plant Diseases / virology
Reoviridae / isolation & purification*
Reverse Transcriptase Polymerase Chain Reaction / methods*
Tenuivirus / isolation & purification*
Time Factors
Virology / methods*