Early prediction of severe acute pancreatitis by urinary β-2 microglobulin/saposin B peak ratios on MALDI-TOF

Clin Chim Acta. 2015 Feb 2:440:115-22. doi: 10.1016/j.cca.2014.11.019. Epub 2014 Nov 21.

Abstract

The current methods for predicting severe acute pancreatitis (severe AP) are either complicated or lack efficient sensitivity and specificity. In this study, a simple and practical approach was developed to predict severe AP by using peak intensity ratio of urinary β-2 microglobulin (B2M) to saposin B (SB) on MALDI-TOF MS. Patients with B2M/SB ratio higher than 1.127 present severe AP symptom with a higher Ranson score, computed tomography (CT) grade and longer hospitalization with a sensitivity of 83.7% and specificity of 74.3%. Label-free quantitative proteomics by nanoLC-MS/MS was applied to urine of severe AP patients and found that severe AP is accompanied with kidney injury and inflammation. The measurement of B2M/SB ratios by MALDI-TOF MS could be a simple, accurate and rapid method to diagnose severe AP as well as to monitor AP progression.

Keywords: Biomarkers; Label-free; Mass spectrometry; Quantitative proteomics; Severe acute pancreatitis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Adult
  • Aged
  • Amino Acid Sequence
  • Case-Control Studies
  • Cholecystitis, Acute / urine
  • Chromatography, Liquid / methods
  • Female
  • Humans
  • Length of Stay
  • Male
  • Middle Aged
  • Molecular Sequence Data
  • Nephritis / urine
  • Pancreatitis / diagnosis*
  • Pancreatitis / urine*
  • Predictive Value of Tests
  • Saposins / urine*
  • Sensitivity and Specificity
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
  • Tandem Mass Spectrometry
  • Tomography, X-Ray Computed
  • beta 2-Microglobulin / urine*

Substances

  • Saposins
  • beta 2-Microglobulin