Oral immunization with recombinant hepatitis E virus antigen displayed on the Lactococcus lactis surface enhances ORF2-specific mucosal and systemic immune responses in mice

Shenyang Gao; Dandan Li; Ying Liu; Enhui Zha; Tiezhong Zhou; Xiqing Yue

doi:10.1016/j.intimp.2014.10.032

Oral immunization with recombinant hepatitis E virus antigen displayed on the Lactococcus lactis surface enhances ORF2-specific mucosal and systemic immune responses in mice

Int Immunopharmacol. 2015 Jan;24(1):140-5. doi: 10.1016/j.intimp.2014.10.032. Epub 2014 Nov 11.

Authors

Shenyang Gao¹, Dandan Li², Ying Liu³, Enhui Zha⁴, Tiezhong Zhou³, Xiqing Yue⁵

Affiliations

¹ Department of Animal Husbandry & Veterinary Medicine, Liaoning Medical University, No. 5-48 Renmin Street, Jinzhou 121001, China; Department of Food Science, Shenyang Agricultural University, No.120 Dongling Road, Shenyang 110161, China.
² Animal Quarantine Lab, Inspection & Quarantine Technology Center of Hainan Entry-Exit Inspection & Quarantine Bureau, Haikou 570000, China.
³ Department of Animal Husbandry & Veterinary Medicine, Liaoning Medical University, No. 5-48 Renmin Street, Jinzhou 121001, China.
⁴ Department of Animal Husbandry & Veterinary Medicine, Liaoning Medical University, No. 5-48 Renmin Street, Jinzhou 121001, China; Department of Food Science and Engineering, Liaoning Medical University, No. 5-48 Renmin Street, Jinzhou 121001, China. Electronic address: 55053287@qq.com.
⁵ Department of Food Science, Shenyang Agricultural University, No.120 Dongling Road, Shenyang 110161, China; Department of Animal Husbandry & Veterinary Medicine, Liaoning Medical University, No. 5-48 Renmin Street, Jinzhou 121001, China.

PMID: 25445956
DOI: 10.1016/j.intimp.2014.10.032

Abstract

Hepatitis E virus (HEV) as a recognized zoonotic pathogen has posed global burden on public health, which is exacerbated by lack of efficient vaccine. In this study, we constructed a recombinant (inaQ-ORF2 gene fusion) Lactococcus lactis (L. lactis) strain NZ3900 that expresses and displays the hepatitis E virus antigen ORF2 utilizing an ice uncleation protein-based anchor system. After oral vaccination of BALB/c mice, significantly higher levels of ORF2-specific mucosal IgA and serum IgG were detected and cellular immunity was also induced. These findings further support that L. lactis-based HEV antigen vaccines could be used for human and animal protection against infection.

Keywords: Hepatitis E virus; Ice nucleation protein; Lactococcus lactis; Oral immunization.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Administration, Oral
Animals
Bacterial Outer Membrane Proteins / genetics
Female
Hepatitis E / immunology
Hepatitis E / prevention & control*
Hepatitis E virus / immunology*
Humans
Immunity, Cellular
Immunity, Mucosal
Immunization
Immunoglobulin A / blood
Immunoglobulin G / blood
Lactococcus lactis / genetics
Mice
Mice, Inbred BALB C
Recombinant Fusion Proteins / genetics
Viral Hepatitis Vaccines / administration & dosage
Viral Hepatitis Vaccines / immunology*
Viral Proteins / genetics
Viral Proteins / immunology*
Zoonoses / immunology
Zoonoses / prevention & control*

Substances

Bacterial Outer Membrane Proteins
Immunoglobulin A
Immunoglobulin G
ORF2 protein, Hepatitis E virus
Recombinant Fusion Proteins
Viral Hepatitis Vaccines
Viral Proteins
ice nucleation protein