Measurement of the biophysical properties of porcine adipose-derived stem cells by a microperfusion system

Jianye Wang; Gang Zhao; Pengfei Zhang; Zhen Wang; Yunhai Zhang; Dayong Gao; Ping Zhou; Yunxia Cao

doi:10.1016/j.cryobiol.2014.10.002

Measurement of the biophysical properties of porcine adipose-derived stem cells by a microperfusion system

Cryobiology. 2014 Dec;69(3):442-50. doi: 10.1016/j.cryobiol.2014.10.002. Epub 2014 Oct 23.

Authors

Jianye Wang¹, Gang Zhao², Pengfei Zhang³, Zhen Wang¹, Yunhai Zhang⁴, Dayong Gao⁵, Ping Zhou⁶, Yunxia Cao⁶

Affiliations

¹ Centre for Biomedical Engineering, Department of Electronic Science and Technology, University of Science and Technology of China, Hefei, Anhui 230027, China.
² Centre for Biomedical Engineering, Department of Electronic Science and Technology, University of Science and Technology of China, Hefei, Anhui 230027, China; Anhui Provincial Engineering Technology Research Center for Biopreservation and Artificial Organs, Hefei, Anhui 230027, China. Electronic address: zhaog@ustc.edu.cn.
³ Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.
⁴ Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China. Electronic address: yunhaizhang@ahau.edu.cn.
⁵ Centre for Biomedical Engineering, Department of Electronic Science and Technology, University of Science and Technology of China, Hefei, Anhui 230027, China; Anhui Provincial Engineering Technology Research Center for Biopreservation and Artificial Organs, Hefei, Anhui 230027, China.
⁶ Anhui Provincial Engineering Technology Research Center for Biopreservation and Artificial Organs, Hefei, Anhui 230027, China.

PMID: 25445459
DOI: 10.1016/j.cryobiol.2014.10.002

Abstract

Adipose-derived stem cells (ADSCs), which are an accessible source of adult stem cells with capacities for self-renewal and differentiation into various cell types, have a promising potential in tissue engineering and regenerative medicine strategies. To meet the clinical demand for ADSCs, cryopreservation has been applied for long-term ADSC preservation. To optimize the addition, removal, freezing, and thawing of cryoprotective agents (CPAs) applied to ADSCs, we measured the transport properties of porcine ADSCs (pADSCs). The cell responses of pADSCs to hypertonic phosphate-buffered saline and common CPAs, dimethyl sulfoxide, ethylene glycol, and glycerol were measured by a microperfusion system at temperatures of 28, 18, 8, and -2°C. We determined the osmotically inactive cell volume (Vb), hydraulic conductivity (Lp), and CPA permeability (Ps) at various temperatures in a two-parameter model. Then, we quantitatively analyzed the effect of temperature on the transport properties of the pADSC membrane. Biophysical parameters were used to optimize CPA addition, removal, and freezing processes to minimize excessive shrinkage of pADSCs during cryopreservation. The biophysical properties of pADSCs have a great potential for effective optimization of cryopreservation procedures.

Keywords: Adipose-derived stem cell; Biophysical properties; Cryopreservation; Microperfusion system.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adipose Tissue / cytology*
Animals
Cell Membrane Permeability
Cells, Cultured
Cryopreservation / instrumentation*
Cryoprotective Agents / metabolism*
Equipment Design
Freezing
Humans
Microscopy / instrumentation
Models, Biological
Osmotic Pressure
Perfusion / instrumentation*
Stem Cells / cytology*
Stem Cells / metabolism
Swine

Substances

Cryoprotective Agents