An on-line HPLC-FRSD system for rapid evaluation of the total antioxidant capacity of Citrus fruits

Food Chem. 2015 Apr 1:172:622-9. doi: 10.1016/j.foodchem.2014.09.121. Epub 2014 Sep 28.

Abstract

An on-line high performance liquid chromatography-free radical scavenging detection (HPLC-FRSD) system was developed for rapidly evaluating the total antioxidant capacity (TAC) of Citrus fruits. With the system, all samples can be analyzed within 5 min. The on-line HPLC-FRSD system has low limits of detection (0.001-0.010 mg mL(-1)) and quantification (0.005-0.020 mg mL(-1)), excellent recovery rate (90.44-115.72%), stability (RSD < 15.80%), reproducibility (RSD < 2%), and precision (RSD < 2%). Using a guard column instead of an analytic column, this new on-line HPLC-FRSD system performed better than the existing on-line HPLC methods in the analysis of the TAC of Citrus. Compared with the conventional off-line 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzthiozoline-6)-sulphonic acid (ABTS) radical scavenging methods, our newly developed system is faster and more robust. The methodology can be a good alternative for analysis TACs of Citrus fruits and potentially for other plants and plant-based products.

Keywords: Citrus fruits; Evaluation; On-line HPLC-FRSD; Total antioxidant capacity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / analysis*
  • Benzothiazoles / analysis*
  • Biphenyl Compounds
  • Chromatography, High Pressure Liquid / methods*
  • Citrus / chemistry*
  • Picrates
  • Plant Extracts / analysis*
  • Reproducibility of Results
  • Sulfonic Acids / analysis*

Substances

  • Antioxidants
  • Benzothiazoles
  • Biphenyl Compounds
  • Picrates
  • Plant Extracts
  • Sulfonic Acids
  • 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
  • 1,1-diphenyl-2-picrylhydrazyl