Purification and identification of dipeptidyl peptidase (DPP) IV inhibitory peptides from the macroalga Palmaria palmata

Food Chem. 2015 Apr 1:172:400-6. doi: 10.1016/j.foodchem.2014.09.083. Epub 2014 Sep 28.

Abstract

Dipeptidyl peptidase (DPP)-IV inhibitory peptides were purified and identified from an aqueous Palmaria palmata protein extract hydrolysed with Corolase PP. The hydrolysate was fractionated by solid phase extraction (SPE) using a C18 matrix followed by semi-preparative reverse phase-high performance liquid chromatography (SP RP-HPLC). IC50 values of 1.47 ± 0.09, 0.54 ± 0.03 and 0.36 ± 0.03 mg/ml were obtained for the hydrolysate, the 25%--acetonitrile (ACN) SPE fraction and the most active SP RP-HPLC peptide fraction (SP RP-HPLC 25_F28), respectively. Thirteen peptide sequences were identified following UPLC-ESI MS/MS analysis of SP RP-HPLC 25_F28. Three novel DPP-IV inhibitory peptides, Ile-Leu-Ala-Pro, Leu-Leu-Ala-Pro and Met-Ala-Gly-Val-Asp-His-Ile, with IC50 values in the range 43-159 μM were identified. The results indicate that P. palmata derived peptides may have potential as functional food ingredients in the prevention and management of type 2 diabetes.

Keywords: Bioactive peptide; Dipeptidyl peptidase IV; Palmaria palmata; Type 2 diabetes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Chromatography, High Pressure Liquid
  • Chromatography, Reverse-Phase
  • Dipeptidyl Peptidase 4 / chemistry
  • Dipeptidyl Peptidase 4 / metabolism
  • Dipeptidyl-Peptidase IV Inhibitors / chemistry*
  • Dipeptidyl-Peptidase IV Inhibitors / isolation & purification
  • Dipeptidyl-Peptidase IV Inhibitors / metabolism
  • Inhibitory Concentration 50
  • Peptides / chemistry*
  • Peptides / isolation & purification
  • Peptides / metabolism
  • Rhodophyta / metabolism*
  • Solid Phase Extraction
  • Spectrometry, Mass, Electrospray Ionization

Substances

  • Dipeptidyl-Peptidase IV Inhibitors
  • Peptides
  • Dipeptidyl Peptidase 4