Construction of transformation system in Penicillium purpurogenum

Ryo Kojima; Teppei Arai; Takafumi Kasumi; Jun Ogihara

doi:10.1016/j.jbiosc.2014.08.016

Construction of transformation system in Penicillium purpurogenum

J Biosci Bioeng. 2015 Mar;119(3):314-6. doi: 10.1016/j.jbiosc.2014.08.016. Epub 2014 Oct 22.

Authors

Ryo Kojima¹, Teppei Arai¹, Takafumi Kasumi¹, Jun Ogihara²

Affiliations

¹ Department of Chemistry and Life Science, Nihon University College of Bioresource Sciences, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
² Department of Chemistry and Life Science, Nihon University College of Bioresource Sciences, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan. Electronic address: j-ogihara@brs.nihon-u.ac.jp.

PMID: 25441444
DOI: 10.1016/j.jbiosc.2014.08.016

Abstract

Penicillium purpurogenum attracts attention in the food industry and biomass degradation. We expressed green fluorescent protein (GFP) with pBPE, a novel vector, and constructed a transformation system for P. purpurogenum. The accumulation of GFP was confirmed by fluorescence microscopy. In future, this system may prove useful for the genetic modification of P. purpurogenum.

Keywords: Green fluorescent protein; Penicillium purpurogenum; Promoter; Protoplasts; Terminator; Transformation.

MeSH terms

Genetic Vectors / genetics
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Microscopy, Fluorescence
Penicillium / genetics*
Penicillium / metabolism
Promoter Regions, Genetic / genetics
Protoplasts / metabolism
Transformation, Bacterial*

Substances

Green Fluorescent Proteins