Separation and purification of amygdalin from thinned bayberry kernels by macroporous adsorption resins

Tao Wang; Shengmin Lu; Qile Xia; Zhongxiang Fang; Stuart Johnson

doi:10.1016/j.jchromb.2014.10.038

Separation and purification of amygdalin from thinned bayberry kernels by macroporous adsorption resins

J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Jan 15:975:52-8. doi: 10.1016/j.jchromb.2014.10.038. Epub 2014 Nov 7.

Authors

Tao Wang¹, Shengmin Lu², Qile Xia³, Zhongxiang Fang⁴, Stuart Johnson⁴

Affiliations

¹ Key Laboratory of Fruits and Vegetables Postharvest and Processing of Zhejiang Province, Institute of Food Science, Zhejiang Academy of Agricultural Science, Hangzhou 310021, PR China; Zhejiang Normal University, Jinhua 321000, PR China.
² Key Laboratory of Fruits and Vegetables Postharvest and Processing of Zhejiang Province, Institute of Food Science, Zhejiang Academy of Agricultural Science, Hangzhou 310021, PR China. Electronic address: lushengmin@hotmail.com.
³ Key Laboratory of Fruits and Vegetables Postharvest and Processing of Zhejiang Province, Institute of Food Science, Zhejiang Academy of Agricultural Science, Hangzhou 310021, PR China.
⁴ Food Science & Technology Program, School of Public Health, Faculty of Health Sciences, International Institute of Agri-Food Security (IIAFS), Curtin University, GPO Box U1987, Perth 6845, WA, Australia.

PMID: 25438243
DOI: 10.1016/j.jchromb.2014.10.038

Abstract

To utilize the low-value thinned bayberry (Myrica rubra Sieb. et Zucc) kernels (TBKs) waste, an efficient method using macroporous adsorption resins (MARs) for separation and purification of amygdalin from TBKs crude extracts was developed. An aqueous crude sample was prepared from a methanol TBK extract, followed by resin separation. A series of MARs were initially screened for adsorption/desorption of amygdalin in the extract, and D101 was selected for characterization and method development. The static adsorption data of amygdalin on D101 was best fitted to the pseudo-second-order kinetics model. The solute affinity toward D101 at 30 °C was described and the equilibrium experimental data were well-fitted to Langmuir and Freundlich isotherms. Through one cycle of dynamic adsorption/desorption, the purity of amygdalin in the extract, determined by HPLC, increased about 17-fold from 4.8% to 82.0%, with 77.9% recovery. The results suggested that D101 resin effectively separate amygdalin from TBKs.

Keywords: Amygdalin; Bayberry; Macroporous adsorption resins (MARs); Purification; Thinned bayberry kernels (TBKs).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adsorption
Amygdalin / isolation & purification*
Antineoplastic Agents, Phytogenic / isolation & purification*
Chromatography, High Pressure Liquid / methods*
Myrica / chemistry*
Plant Extracts / chemistry*
Porosity
Resins, Synthetic / chemistry*

Substances

Antineoplastic Agents, Phytogenic
Plant Extracts
Resins, Synthetic
Amygdalin