Genotoxic effects of CdS quantum dots and Ag2S nanoparticles in fish cell lines (RTG-2)

Mutat Res Genet Toxicol Environ Mutagen. 2014 Dec:775-776:89-93. doi: 10.1016/j.mrgentox.2014.09.003. Epub 2014 Sep 16.

Abstract

The increasing use of nanotechnologies will lead to significant releases of engineered nanoparticles into the aquatic environment, where their impact is still poorly characterized. In the present paper, the genotoxic and cytotoxic properties of CdS quantum dots (QDs) and silver sulphide (Ag2S) coated with methyl polyethylene glycol (M-PEG) were investigated in a rainbow trout cell line (RTG-2). The results showed that CdS QDs were highly cytotoxic at high concentrations (10 and 50μg/ml), and exhibited a concentration-dependent genotoxicity in the sub-toxic range (0.01-1μg/ml) after 24h exposure. Ag2S showed neither genotoxic nor cytotoxic effects.

Keywords: Cadmium; DNA damage; Fish cell culture; Nanoparticles; Quantum dots; Silver.

MeSH terms

  • Animals
  • Cadmium Compounds / chemical synthesis
  • Cadmium Compounds / chemistry
  • Cadmium Compounds / toxicity*
  • Cell Line
  • Cell Survival / drug effects
  • DNA Damage*
  • Dose-Response Relationship, Drug
  • Gonads / drug effects
  • Metal Nanoparticles / chemistry
  • Metal Nanoparticles / toxicity
  • Models, Animal
  • Oncorhynchus mykiss*
  • Polyethylene Glycols / chemistry
  • Silver Compounds / chemical synthesis
  • Silver Compounds / toxicity*
  • Sulfides / chemical synthesis
  • Sulfides / chemistry
  • Sulfides / toxicity*

Substances

  • Cadmium Compounds
  • Silver Compounds
  • Sulfides
  • cadmium sulfide
  • Polyethylene Glycols
  • silver sulfide