Cytotoxicity induced by nanobacteria and nanohydroxyapatites in human choriocarcinoma cells

Mingjun Zhang; Jinmei Yang; Jing Shu; Changhong Fu; Shengnan Liu; Ge Xu; Dechun Zhang

doi:10.1186/1556-276X-9-616

Cytotoxicity induced by nanobacteria and nanohydroxyapatites in human choriocarcinoma cells

Nanoscale Res Lett. 2014 Nov 14;9(1):616. doi: 10.1186/1556-276X-9-616. eCollection 2014.

Authors

Mingjun Zhang¹, Jinmei Yang¹, Jing Shu¹, Changhong Fu², Shengnan Liu¹, Ge Xu³, Dechun Zhang¹

Affiliations

¹ Molecular Medicine and Tumor Research Center, Chongqing Medical University, Medical College Road, Yuzhong District, Chongqing 400016, People's Republic of China.
² The First People's Hospital of Jiulongpo District, Chongqing, People's Republic of China.
³ Electron Microscopy Group, Department of Life Science, Chongqing Medical University, Chongqing, People's Republic of China.

Abstract

We explored the cytotoxic effects of nanobacteria (NB) and nanohydroxyapatites (nHAPs) against human choriocarcinoma cells (JAR) and the mechanisms of action underlying their cytotoxicity. JAR cells were co-cultured with NB and nHAPs for 48 h, and ultrastructural changes were more readily induced by NB than nHAPs. Autophagy in the plasma of JAR cells were observed in the NB group. The rate of apoptosis induced by NB was higher than that for nHAPs. The expression of Bax and FasR proteins in the NB group was stronger than that for the nHAP group. NB probably resulted in autophagic formation. Apoptosis was possibly activated via FasL binding to the FasR signaling pathway.

Keywords: Apoptosis; Autophagy; Cytotoxicity; Human choriocarcinoma (JAR) cells; Nanobacteria (NB); Nanohydroxyapatites (nHAPs).