Long-term reduction of T-cell intracellular antigens reveals a transcriptome associated with extracellular matrix and cell adhesion components

PLoS One. 2014 Nov 18;9(11):e113141. doi: 10.1371/journal.pone.0113141. eCollection 2014.

Abstract

Knockdown of T-cell intracellular antigens TIA1 and TIAR contributes to a cellular phenotype characterised by uncontrolled proliferation and tumorigenesis. Massive-scale poly(A+) RNA sequencing of TIA1 or TIAR-knocked down HeLa cells reveals transcriptome signatures comprising genes and functional categories potentially able to modulate several aspects of membrane dynamics associated with extracellular matrix and focal/cell adhesion events. The transcriptomic heterogeneity is the result of differentially expressed genes and RNA isoforms generated by alternative splicing and/or promoter usage. These results suggest a role for TIA proteins in the regulation and/or modulation of cellular homeostasis related to focal/cell adhesion, extracellular matrix and membrane and cytoskeleton dynamics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Adhesion / physiology*
  • Extracellular Matrix / physiology*
  • Fluorescent Antibody Technique
  • Gene Expression Profiling
  • Gene Knockdown Techniques
  • Gene Library
  • HeLa Cells
  • Humans
  • Luciferases
  • Microscopy, Fluorescence
  • Poly(A)-Binding Proteins / genetics*
  • Sequence Analysis, RNA
  • T-Cell Intracellular Antigen-1
  • Transcriptome / physiology*

Substances

  • Poly(A)-Binding Proteins
  • T-Cell Intracellular Antigen-1
  • TIA1 protein, human
  • Luciferases

Grants and funding

This work was supported by grants from Ministerio de Economía y Competitividad-FEDER (BFU2008-00354 and BFU2011-29653). The CBMSO receives an institutional grant from Fundación Ramón Areces, Spain. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.