Multi-isotype antibody responses against the multimeric Salmonella Typhi recombinant hemolysin E antigen

Microbiol Immunol. 2015 Jan;59(1):43-7. doi: 10.1111/1348-0421.12211.

Abstract

The detection and measurement of different antibody isotypes in the serum provide valuable indicators of the different stages of typhoid infection. Here, the ability of S. Typhi recombinant hemolysin E (HlyE) to detect multi-isotype antibody responses in sera of patients with typhoid and paratyphoid A was investigated using an indirect antibody immunoassay. Nanogram amounts of HlyE were found to be sufficient for detection of IgG and IgA isotypes and, in a study of individuals' sera (n = 100), the immunoassay was able to distinguish between typhoid and non-typhoid sera. The overall sensitivity, specificity and efficiency of the ELISA were 70% (39/56), 100% (44/44) and 83% respectively.

Keywords: ELISA; Salmonella Typhi; antibody isotypes; recombinant hemolysin E antigen.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Bacterial / blood*
  • Antigens, Bacterial / genetics
  • Antigens, Bacterial / immunology*
  • Diagnostic Tests, Routine / methods*
  • Enzyme-Linked Immunosorbent Assay / methods
  • Hemolysin Proteins / genetics
  • Hemolysin Proteins / immunology*
  • Hepatitis B e Antigens
  • Humans
  • Immunoglobulin A / blood
  • Immunoglobulin G / blood
  • Paratyphoid Fever / diagnosis
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology
  • Salmonella typhi / genetics
  • Salmonella typhi / immunology*
  • Sensitivity and Specificity
  • Serologic Tests / methods
  • Typhoid Fever / diagnosis*

Substances

  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Hemolysin Proteins
  • Hepatitis B e Antigens
  • HlyE protein, Salmonella enterica
  • Immunoglobulin A
  • Immunoglobulin G
  • Recombinant Proteins