Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of Vibrio cholerae

J Clin Microbiol. 2015 Jan;53(1):329-31. doi: 10.1128/JCM.02666-14. Epub 2014 Nov 12.

Abstract

We evaluated the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MS) for the identification of Vibrio cholerae. MS identified all 42 isolates of V. cholerae O1 and O139 and 7 of 9 non-O1/O139 isolates. MS correctly discriminated between all Aeromonas and V. cholerae isolates. Overall, MS performed as well as or better than biochemical methods.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics
  • Bacterial Typing Techniques / methods*
  • Cholera / diagnosis*
  • Cholera / microbiology
  • DNA, Bacterial / analysis
  • DNA, Bacterial / genetics
  • Humans
  • Molecular Typing / methods*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
  • Vibrio cholerae / chemistry*
  • Vibrio cholerae / classification

Substances

  • Bacterial Proteins
  • DNA, Bacterial