An acidic pectin lyase from Aspergillus niger with favourable efficiency in fruit juice clarification

Lett Appl Microbiol. 2015 Feb;60(2):181-187. doi: 10.1111/lam.12357. Epub 2014 Dec 15.

Abstract

The pectin lyase gene pnl-zj5a from Aspergillus niger ZJ5 was identified and expressed in Pichia pastoris. PNL-ZJ5A was purified by ultrafiltration, anion exchange and gel chromatography. The Km and Vmax values determined using citrus pectin were 0.66 mg ml(-1) and 32.6 μmol min(-1) mg(-1) , respectively. PNL-ZJ5A exhibited optimal activity at 43°C and retained activity over 25-50°C. PNL-ZJ5A was optimally active at pH 5 and effective in apple juice clarification. Compared with controls, PNL-ZJ5A increased the fruit juice yield significantly. Furthermore, PNL-ZJ5A reduced the viscosity of apple juice by 38.8% and increased its transmittance by 86.3%. PNL-ZJ5A combined with a commercial pectin esterase resulted in higher juice volume.

Keywords: Aspergillus niger; Pichia pastoris; juice clarification; pectin lyase; viscosity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aspergillus niger / enzymology*
  • Aspergillus niger / genetics
  • Beverages*
  • Carboxylic Ester Hydrolases / metabolism
  • Cloning, Molecular
  • Food Handling / methods*
  • Fruit*
  • Fungal Proteins / genetics
  • Fungal Proteins / isolation & purification
  • Fungal Proteins / metabolism
  • Genes, Fungal
  • Hydrogen-Ion Concentration
  • Kinetics
  • Malus*
  • Pectins / metabolism
  • Pichia / genetics
  • Polysaccharide-Lyases / genetics
  • Polysaccharide-Lyases / isolation & purification
  • Polysaccharide-Lyases / metabolism*
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / metabolism
  • Substrate Specificity
  • Viscosity

Substances

  • Fungal Proteins
  • Recombinant Proteins
  • Pectins
  • Carboxylic Ester Hydrolases
  • pectinesterase
  • Polysaccharide-Lyases
  • pectin lyase