Needles of Pinus halepensis as biomonitors of bioaerosol emissions

PLoS One. 2014 Nov 7;9(11):e112182. doi: 10.1371/journal.pone.0112182. eCollection 2014.

Abstract

We propose using the surface of pine trees needles to biomonitor the bioaerosol emissions at a composting plant. Measurements were based on 16S rRNA gene copy numbers of Saccharopolyspora rectivirgula, a bioindicator of composting plant emissions. A sampling plan was established based on 29 samples around the emission source. The abundance of 16S rRNA gene copies of S. rectivirgula per gram of Pinus halepensis needles varied from 104 to 102 as a function of the distance. The signal reached the background level at distances around the composting plant ranging from 2 km to more than 5.4 km, depending on the local topography and average wind directions. From these values, the impacted area around the source of bioaerosols was mapped.

MeSH terms

  • Aerosols
  • Air Microbiology*
  • Environmental Monitoring / methods*
  • Gene Dosage
  • Pinus / microbiology*
  • Plant Leaves / microbiology
  • RNA, Ribosomal, 16S / genetics
  • Saccharopolyspora / genetics
  • Saccharopolyspora / isolation & purification
  • Time Factors

Substances

  • Aerosols
  • RNA, Ribosomal, 16S

Grants and funding

The author(s) received no specific funding for this work.