Abstract
Tremella fuciformis yeast-like conidium (YLC) cells were transformed by co-cultivation with Agrobacterium cells harboring the hepatitis B surface antigen (HBsAg) gene construct under the control of the CaMV35S promoter. Integration of HBsAg DNA into the YLC genome was confirmed by PCR and dot-blot hybridization. Immunoblotting verified expression of the recombinant protein. Oral administration of YLC cells expressing HBsAg in mice significantly increased anti-HBsAg antibody titer levels using a double prime-boost strategy that combined parenteral and oral HBsAg boosters.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Administration, Oral
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Animals
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Basidiomycota / genetics*
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Drug Carriers / administration & dosage*
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Genetic Vectors
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Hepatitis B Antibodies / blood
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Hepatitis B Surface Antigens / genetics
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Hepatitis B Surface Antigens / immunology*
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Hepatitis B Vaccines / administration & dosage
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Hepatitis B Vaccines / genetics
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Hepatitis B Vaccines / immunology*
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Mice
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Recombinant Proteins / genetics
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Recombinant Proteins / immunology
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Spores, Fungal / genetics
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Vaccination / methods*
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Vaccines, Synthetic / administration & dosage
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Vaccines, Synthetic / genetics
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Vaccines, Synthetic / immunology
Substances
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Drug Carriers
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Hepatitis B Antibodies
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Hepatitis B Surface Antigens
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Hepatitis B Vaccines
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Recombinant Proteins
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Vaccines, Synthetic