Nanoparticle-protein conjugates are promising probes for biological diagnostics as well as versatile building blocks for nanotechnology. Here we demonstrate a facile method to prepare nanoparticles bearing discrete numbers of BSA simply by physical adsorption and electrophoretic isolation, in which the specific amphiphilic properties of BSA play important roles and the number of adsorbed BSA molecules can also be manipulated by tuning the coating extent of nanoparticles by amphiphilic polymer.
Keywords: BSA; discrete conjugate; hydrophobic interaction; nanoparticle; physical adsorption.