"Perfecting" pure shift HSQC: full homodecoupling for accurate and precise determination of heteronuclear couplings

Chem Commun (Camb). 2014 Dec 25;50(99):15702-5. doi: 10.1039/c4cc04217d. Epub 2014 Oct 31.

Abstract

Fully homodecoupled HSQC spectra can be obtained through the use of a new pulse sequence element, "perfectBIRD". By way of illustration, we show that perfectBIRD decoupling allows one-bond residual dipolar couplings (RDCs), which provide important NMR restraints for structure elucidation, to be measured with outstanding precision, even in methylene groups.