LIN28B promotes colon cancer migration and recurrence

PLoS One. 2014 Oct 31;9(10):e109169. doi: 10.1371/journal.pone.0109169. eCollection 2014.

Abstract

LIN28B is involved in "stemness" and tumourigenesis by negatively regulating the maturation of let-7 microRNA family members. In this study, we showed that LIN28B expression promotes migration and recurrence of colon cancer. Immunohistochemistry and reverse-transcription polymerase chain reactions were performed to detect LIN28B expression in colon cancer tissue microarrays, paraffin-embedded surgical resected tissues and cancer cells. Loss-of-function, migration and proliferation analyses were performed to delineate the potential roles of LIN28B in colon cancer. LIN28B was upregulated in colon cancer tissue compared to normal mucosa, and its overexpression correlated with reduced patient survival and increased tumour recurrence. LIN28B suppression inhibited the migration of SW480 colon cancer cells and facilitated the cytotoxicity induced by oxaliplatin in SW480 and HCT116 colon cancer cells. In conclusion, LIN28B overexpression contributes to colon tumourigenesis, and LIN28B may serve as a diagnostic tool and therapeutic target for colon cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / pharmacology
  • Cell Line, Tumor
  • Cell Movement
  • Colonic Neoplasms / metabolism
  • Colonic Neoplasms / mortality*
  • Colonic Neoplasms / pathology*
  • Colonic Neoplasms / surgery
  • Female
  • HCT116 Cells / drug effects
  • HCT116 Cells / pathology
  • Humans
  • Intestinal Mucosa / metabolism
  • Male
  • Middle Aged
  • Neoplasm Recurrence, Local
  • Organoplatinum Compounds / pharmacology
  • Oxaliplatin
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / metabolism*
  • Reference Values
  • Tissue Array Analysis
  • Up-Regulation

Substances

  • Antineoplastic Agents
  • LIN28B protein, human
  • Organoplatinum Compounds
  • RNA-Binding Proteins
  • Oxaliplatin

Grants and funding

This work was supported by grants from the Department of Public Health of Sichuan Province (110167). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.