Transactivation of large ribozymes

Matthew B Martin; Thomas L Leeper; Frank J Schmidt

doi:10.1007/978-1-4939-1896-6_5

Transactivation of large ribozymes

Methods Mol Biol. 2015:1240:57-62. doi: 10.1007/978-1-4939-1896-6_5.

Authors

Matthew B Martin¹, Thomas L Leeper, Frank J Schmidt

Affiliation

¹ Department of Biochemistry, University of Missouri, 117 Schweitzer Hall, 103A Schlundt Annex, Columbia, MO, 65211, USA.

PMID: 25352137
DOI: 10.1007/978-1-4939-1896-6_5

Abstract

The domains of large ribozymes are composed of secondary structural elements that fold independently in solution. Interactions between these structures contribute to the overall structure of the ribozyme. These elements can also be deleted, reducing catalytic activity, and their structure-function relationships determined by assaying the ability of variant elements to activate the deleted ribozyme in trans. This is illustrated for an unusual loop structure from Bacillus subtilis Ribonuclease P RNA.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Bacillus subtilis / enzymology
Base Sequence
Molecular Biology / methods*
Molecular Sequence Data
Mutation / genetics
RNA, Catalytic / chemistry
RNA, Catalytic / genetics*
RNA, Catalytic / metabolism
Ribonuclease P / chemistry
Ribonuclease P / genetics
Ribonuclease P / metabolism
Transcriptional Activation / genetics*

Substances

RNA, Catalytic
Ribonuclease P