ROS-mediated cytotoxic effect of copper(II) hydrazone complexes against human glioma cells

Molecules. 2014 Oct 27;19(11):17202-20. doi: 10.3390/molecules191117202.

Abstract

2-Acetylpyridine acetylhydrazone (H2AcMe), 2-benzoylpyridine acetylhydrazone (H2BzMe) and complexes [Cu(H2AcMe)Cl2] (1) and [Cu(H2BzMe)Cl2] (2) were assayed for their cytotoxicity against wild type p53 U87 and mutant p53 T98 glioma cells, and against MRC-5 fibroblast cells. Compounds 1 and 2 proved to be more active than the corresponding hydrazones against U87, but not against T98 cells. Compound 1 induced higher levels of ROS than H2AcMe in both glioma cell lines. H2AcMe and 1 induced lower levels of ROS in MRC5 than in U87 cells. Compound 2 induced lower levels of ROS in MRC5 than in T98 cells. The cytotoxic effect of 1 in U87 cells could be related to its ability to provoke the release of ROS, suggesting that the cytotoxicity of 1 might be somehow p53 dependent.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Cell Line, Tumor
  • Copper / pharmacology*
  • Fibroblasts / drug effects
  • Glioma / drug therapy*
  • Glioma / metabolism
  • Humans
  • Hydrazones / pharmacology*
  • Reactive Oxygen Species / metabolism*
  • Tumor Suppressor Protein p53 / metabolism

Substances

  • Hydrazones
  • Reactive Oxygen Species
  • Tumor Suppressor Protein p53
  • Copper