New records of genera and species of spider wasps (Hymenoptera: Pompilidae) from Colombia are provided. Agenioideus, Cryptocheilus, Evagetes, Mystacagenia, and Xerochares are newly recorded genera from Colombia. Nineteen species are first recorded from Colombia: Aimatocarevitrea (Fox); Ageniellaazteca (Cameron); Ageniellacurtipinus (Cameron); Ageniellafallax (Arlé); Ageniellahirsuta Banks; Ageniellapilifrons (Cameron); Ageniellapretiosa Banks; Ageniellasanguinolenta (Smith); Ageniellazeteki (Banks); Agenioideusbirkmanni (Banks); Aporus (Aporus) cuzco Evans; Aporus (Cosmiaporus) diverticulus (Fox); Aporus (Notoplaniceps) canescens Smith; Euplanicepsexilis (Banks); Euplanicepsherbertii (Fox); Irenangelusclarus Evans; Mystacageniabellula Evans; Phanochilusnobilitatus (Smith) and Xerocharesexpulsus Schulz. The following species and genera have their occurence ranges expanded for South America: Ageniellaazteca (Cameron); Ageniellazeteki (Banks); Agenioideusbirkmanni (Banks); and Xerocharesexpulsus Schulz; Cryptocheilus Panzer; and Xerochares Evans.
Keywords: Neotropical; Pompilidae; South America; new record.