Abstract
Dysregulation of miRNAs is involved in osteosarcoma (OS). Here, we demonstrate that miR-382 is decreased in specimens of OS patients with a poor chemoresponse compared to those with a good chemoresponse. In addition, our clinical data show that decreased miR-382 was associated with poor survival in OS patients. Overexpression of miR-382 inhibited cell growth and chemoresistance by targeting KLF12 and HIPK3, respectively. In contrast, inhibition of miR-382 or overexpression of target genes stimulated OS cell growth and chemoresistance both in vitro and in vivo. Taken together, these findings suggest that miR-382 is a tumor suppressor miRNA and induction of miR-382 is a potential strategy to inhibit OS progression.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Apoptosis / genetics
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Bone Neoplasms / genetics
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Bone Neoplasms / mortality
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Bone Neoplasms / pathology
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Cell Line, Tumor
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Cell Proliferation / genetics
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Drug Resistance, Neoplasm / genetics*
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Female
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Gene Expression Regulation, Neoplastic
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Humans
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Intracellular Signaling Peptides and Proteins / biosynthesis*
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Intracellular Signaling Peptides and Proteins / genetics
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Kruppel-Like Transcription Factors / biosynthesis*
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Kruppel-Like Transcription Factors / genetics
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Mice
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Mice, Nude
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MicroRNAs / antagonists & inhibitors
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MicroRNAs / biosynthesis
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MicroRNAs / genetics*
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Osteosarcoma / drug therapy*
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Osteosarcoma / genetics
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Osteosarcoma / mortality
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Osteosarcoma / pathology
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Protein Serine-Threonine Kinases / biosynthesis*
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Protein Serine-Threonine Kinases / genetics
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Treatment Outcome
Substances
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Intracellular Signaling Peptides and Proteins
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KLF12 protein, human
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Kruppel-Like Transcription Factors
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MIRN382 microRNA, human
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MicroRNAs
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HIPK3 protein, human
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Protein Serine-Threonine Kinases