Whole-genome sequencing of Burkholderia pseudomallei isolates from an unusual melioidosis case identifies a polyclonal infection with the same multilocus sequence type

J Clin Microbiol. 2015 Jan;53(1):282-6. doi: 10.1128/JCM.02560-14. Epub 2014 Oct 22.

Abstract

Twelve Burkholderia pseudomallei isolates collected over a 32-month period from a patient with chronic melioidosis demonstrated identical multilocus sequence types (STs). However, whole-genome sequencing suggests a polyclonal infection. This study is the first to report a mixed infection with the same ST.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bacterial Typing Techniques / methods*
  • Burkholderia pseudomallei / classification*
  • Burkholderia pseudomallei / genetics*
  • Genome, Bacterial / genetics*
  • Humans
  • Male
  • Melioidosis / microbiology*
  • Middle Aged
  • Multilocus Sequence Typing / methods*
  • Phylogeny