This study was purposed to investigate the RHCE gene polymorphisms in Chinese Jiangsu Han blood donors with and without RHD gene among serologic RhD negative population. PCR with sequence-specific primers (PCR-SSP) was used to detect RHCE genotype in 337 serologic RhD negative, RHD gene positive donors. The RHD gene-specific polymorphisms were also determined by PCR-SSP in these donors. The results showed that among 337 serologic RhD negative, RHD gene positive donor 20 were RHCE*C/C, 62 RHCE*C/c, 24 RHCE*c/c, 25 RHCE*E/e, and 81 RHCE*e/e; the allele frequencies for RHCE*C and RHCE*c were 0.4811 and 0.5189, respectively; and for RHCE*E and RHCE*e 0.1179 and 0.8821. Among 231 RHD gene negative donors, 3 were RHCE*C/C, 34 RHCE*C/c, 194 RHCE*c/c, 15 RHCE*E/e, 216 RHCE*e/e; the allele frequencies for RHCE*C and RHCE*c were 0.0866 and 0.9134, respectively; and allele frequencies for RHCE*E and RHCE*e were 0.0325 and 0.9675, respectively. It is concluded that the most prevalent allele of RHCE gene was RHCE*ccee in Chinese Han Jiangsu RHD gene negative population. There is statistical difference in RHCE genotype distribution among serologic RhD negative population with and without RHD gene.