Abstract
Condensin is enriched in the pericentromere of budding yeast chromosomes where it is constrained to the spindle axis in metaphase. Pericentric condensin contributes to chromatin compaction, resistance to microtubule-based spindle forces, and spindle length and variance regulation. Condensin is clustered along the spindle axis in a heterogeneous fashion. We demonstrate that pericentric enrichment of condensin is mediated by interactions with transfer ribonucleic acid (tRNA) genes and their regulatory factors. This recruitment is important for generating axial tension on the pericentromere and coordinating movement between pericentromeres from different chromosomes. The interaction between condensin and tRNA genes in the pericentromere reveals a feature of yeast centromeres that has profound implications for the function and evolution of mitotic segregation mechanisms.
© 2014 Snider et al.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Adenosine Triphosphatases / analysis
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Adenosine Triphosphatases / metabolism*
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Centrosome / metabolism
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Centrosome / ultrastructure
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Chromatin / metabolism*
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Chromatin / ultrastructure
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DNA-Binding Proteins / analysis
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DNA-Binding Proteins / metabolism*
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Hydro-Lyases / analysis
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Hydro-Lyases / metabolism
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Hydro-Lyases / physiology*
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Kinetochores / metabolism
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Microtubule-Associated Proteins / analysis
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Microtubule-Associated Proteins / metabolism
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Microtubule-Associated Proteins / physiology*
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Mitosis / physiology*
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Multiprotein Complexes / analysis
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Multiprotein Complexes / metabolism*
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RNA, Transfer / genetics*
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Ribonucleoproteins, Small Nuclear / analysis
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Ribonucleoproteins, Small Nuclear / metabolism
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Ribonucleoproteins, Small Nuclear / physiology*
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Saccharomyces cerevisiae / cytology*
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Saccharomyces cerevisiae Proteins / analysis
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Saccharomyces cerevisiae Proteins / metabolism
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Saccharomyces cerevisiae Proteins / physiology*
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Spindle Apparatus / metabolism*
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Spindle Apparatus / ultrastructure
Substances
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Chromatin
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DNA-Binding Proteins
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Microtubule-Associated Proteins
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Multiprotein Complexes
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Ribonucleoproteins, Small Nuclear
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Saccharomyces cerevisiae Proteins
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condensin complexes
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RNA, Transfer
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Adenosine Triphosphatases
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Hydro-Lyases
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CBF5 protein, S cerevisiae