Screening rectal swabs for carbapenemase genes

Anneke van der Zee; Lieuwe Roorda; Gerda Bosman; Jacobus M Ossewaarde

doi:10.1128/JCM.02256-14

Screening rectal swabs for carbapenemase genes

J Clin Microbiol. 2014 Dec;52(12):4401-3. doi: 10.1128/JCM.02256-14. Epub 2014 Oct 15.

Authors

Anneke van der Zee¹, Lieuwe Roorda², Gerda Bosman², Jacobus M Ossewaarde³

Affiliations

¹ Maasstad Laboratory, Molecular Diagnostics Unit, Rotterdam, The Netherlands zeea@maasstadziekenhuis.nl.
² Maasstad Laboratory, Molecular Diagnostics Unit, Rotterdam, The Netherlands.
³ Maasstad Laboratory, Molecular Diagnostics Unit, Rotterdam, The Netherlands Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Centre, Rotterdam, The Netherlands.

Abstract

In an outbreak setting, we screened 16,296 samples from 3,644 patients by PCR for the presence of blaOXA-48, blaVIM, blaIMP, blaNDM, and blaKPC. The blaOXA-48 gene was found in samples from 43 patients infected with 9 different species of Enterobacteriaceae. Five patients had Pseudomonas aeruginosa isolates containing blaVIM. The negative predictive value of screening was 100%, and the positive predictive value was 86%.

Publication types

Evaluation Study

MeSH terms

Bacterial Proteins / analysis*
Bacterial Proteins / genetics
Disease Outbreaks
Enterobacteriaceae / enzymology*
Enterobacteriaceae / genetics
Gram-Negative Bacterial Infections / diagnosis
Gram-Negative Bacterial Infections / epidemiology
Gram-Negative Bacterial Infections / microbiology*
Humans
Mass Screening / methods*
Molecular Diagnostic Techniques / methods
Polymerase Chain Reaction / methods*
Predictive Value of Tests
Pseudomonas aeruginosa / enzymology*
Pseudomonas aeruginosa / genetics
Rectum / microbiology*
beta-Lactamases / analysis*
beta-Lactamases / genetics

Substances

Bacterial Proteins
beta-Lactamases
carbapenemase