A comparative in vitro antioxidant potential profile of extracts from different parts of Fagonia cretica

Pervaiz Iqbal; Dildar Ahmed; Muhammad Nadeem Asghar

doi:10.1016/S1995-7645(14)60277-7

A comparative in vitro antioxidant potential profile of extracts from different parts of Fagonia cretica

Asian Pac J Trop Med. 2014 Sep:7S1:S473-80. doi: 10.1016/S1995-7645(14)60277-7.

Authors

Pervaiz Iqbal¹, Dildar Ahmed¹, Muhammad Nadeem Asghar²

Affiliations

¹ Department of Chemistry, Forman Christian College (A Chartered University), Lahore 54600, Pakistan.
² Department of Chemistry, Forman Christian College (A Chartered University), Lahore 54600, Pakistan. Electronic address: nadeemasghar@fccollege.edu.pk.

PMID: 25312170
DOI: 10.1016/S1995-7645(14)60277-7

Abstract

Objective: To evaluate antioxidant and radical scavenging activities of organic extracts from fruit, roots and aerial parts of Fagonia cretica.

Methods: Shed dried and powdered plant parts were initially extracted in methanol and subsequently partitioned in n-hexane, chloroform, ethyl acetate and 1-butanol successively. Antioxidant and radical scavenging potential of the methanol extracts and the fractions of each part were evaluated using total phenolic contents (TPC) and total flavonoid contents (TFC), 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation radicals scavenging, reducing power (potassium ferricyanide-trichloroacetic acid system), ferric ion reducing antioxidant potential, lipid peroxidation inhibition activity (linoleic acid system) and total antioxidant activity (phosphomolybdate) assays.

Results: TPC and TFC values for methanol extracts and various fractions ranged from 0.23-4.30 mg/L gallic acid equivalents and from 30-545 mg/L rutin equivalents, respectively. Overall, methanol extracts and all the fractions of root and aerial parts showed higher TPC and TFC values. Methanol extracts and aqueous fractions of root and aerial parts and the n-butanol fraction of root showed lower EC50 values for 2,2-diphenyl-1-picrylhydrazyl scavenging than the other plant extracts. The 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging, total antioxidant potential and ferric ion reducing antioxidant potential values confirmed the presence of potent antioxidant principles in the methanol extract of roots. In general, all the extracts/fractions and especially those of root showed high antioxidant and radical scavenging activities.

Conclusions: The crude methanol extract of root can be explored further for in vivo studies. This study revealed the potent antioxidant potential of Fagonia cretica and its prospective efficacy against various reactive oxygen species-mediated diseases.

Keywords: ABTS(•+); Antioxidant potential; Fagonia cretica; TFC; TPC.