Multiplex SNP detection in multiple codons for accurate drug therapy

Chem Commun (Camb). 2014 Dec 4;50(93):14585-8. doi: 10.1039/c4cc06625a.

Abstract

Multiplex SNP discrimination in the multiple codons of the genomic DNA is demonstrated by applying controller DNA technology (CDT) to MDR-TB 9G DNAChips. CDT allowed the efficient detection of 20 SNPs in five codons of the genomic DNA in 40 min. CDT could distinguish SNP targets to as low as 1 copy of the genomic DNA. 100% agreement with the sequencing analysis of clinical samples highlighted the clinical applicability of MDR-TB 9G DNAChips.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Codon / genetics*
  • DNA / genetics
  • Oligonucleotide Array Sequence Analysis
  • Pharmaceutical Preparations*
  • Pharmacogenetics / methods*
  • Polymorphism, Single Nucleotide / genetics*
  • Sequence Analysis, DNA / methods*

Substances

  • Codon
  • Pharmaceutical Preparations
  • DNA