Laboratory evaluation of three rapid diagnostic tests for dual detection of HIV and Treponema pallidum antibodies

Romney M Humphries; Jennifer S Woo; Jun Ho Chung; Anita Sokovic; Claire C Bristow; Jeffrey D Klausner

doi:10.1128/JCM.02468-14

Laboratory evaluation of three rapid diagnostic tests for dual detection of HIV and Treponema pallidum antibodies

J Clin Microbiol. 2014 Dec;52(12):4394-7. doi: 10.1128/JCM.02468-14. Epub 2014 Oct 8.

Authors

Romney M Humphries¹, Jennifer S Woo², Jun Ho Chung², Anita Sokovic³, Claire C Bristow³, Jeffrey D Klausner³

Affiliations

¹ Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, California, USA rhumphries@mednet.ucla.edu.
² Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, California, USA.
³ Department of Infectious Diseases, David Geffen School of Medicine at UCLA, Los Angeles, California, USA.

Abstract

The performance of three research-use-only, dual HIV and syphilis rapid diagnostic tests (RDTs) was evaluated for 150 patient serum samples and compared to reference HIV and Treponema pallidum antibody detection methods. The RDTs performed comparably, with sensitivities of 93 to 99% and specificities of 97 to 100%. The kappa statistic between the RDTs was 0.95.

Publication types

Comparative Study
Evaluation Study

MeSH terms

Adult
Antibodies, Bacterial / blood*
Diagnostic Tests, Routine / methods*
Female
HIV / immunology
HIV Antibodies / blood*
HIV Infections / diagnosis*
HIV Infections / virology
Humans
Immunoassay / methods
Male
Sensitivity and Specificity
Syphilis / diagnosis*
Syphilis / microbiology
Treponema pallidum / immunology

Substances

Antibodies, Bacterial
HIV Antibodies