NMR study of non-structural proteins--part II: (1)H, (13)C, (15)N backbone and side-chain resonance assignment of macro domain from Venezuelan equine encephalitis virus (VEEV)

Biomol NMR Assign. 2015 Oct;9(2):247-51. doi: 10.1007/s12104-014-9584-9. Epub 2014 Oct 8.

Abstract

Macro domains consist of 130-190 amino acid residues and appear to be highly conserved in all kingdoms of life. Intense research on this field has shown that macro domains bind ADP-ribose and other similar molecules, but their exact function still remains intangible. Macro domains are highly conserved in the Alphavirus genus and the Venezuelan equine encephalitis virus (VEEV) is a member of this genus that causes fatal encephalitis to equines and humans. In this study we report the high yield recombinant expression and preliminary solution NMR study of the macro domain of VEEV. An almost complete sequence-specific assignment of its (1)H, (15)N and (13)C resonances was obtained and its secondary structure predicted by TALOS+. The protein shows a unique mixed α/β-fold.

Keywords: ADP-ribose-binding module; Alphavirus; NMR spectroscopy; Recombinant protein expression; Venezuelan equine encephalitis virus; Viral macro domains.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Carbon-13 Magnetic Resonance Spectroscopy*
  • Encephalitis Virus, Venezuelan Equine / metabolism*
  • Molecular Sequence Data
  • Nitrogen Isotopes
  • Nuclear Magnetic Resonance, Biomolecular*
  • Protein Structure, Tertiary
  • Proton Magnetic Resonance Spectroscopy*
  • Sequence Alignment
  • Viral Nonstructural Proteins / chemistry*

Substances

  • Nitrogen Isotopes
  • Viral Nonstructural Proteins